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Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.