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说明书：5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution说明书（点击查看下载）
5-Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) (cat. no. C401A) is supplied as a 10 mM solution in TE buffer. 5-Aminoallyl-dUTP is also available in lyophilized form (cat. no. C401B).
5-Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
5-Aminoallyl-dUTP可以在Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I等作用下，合成含amine的DNA片段，这些片段可以和任何带amine活性的荧光染料，生物素或是抗原反应生成需要的核酸标记探针。
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Specifications:
Concentration: 10 mM solution in TE buffer
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Applications
Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.
Applied BioProbes 最新推出一系列荧光素和生物素标记dUTP和UTP探针。这些探针可广泛应用于FISH, TUNEL, DNA microarray等应用。我们的探针采用荧光亮度强，光稳定性好的Andy Fluor染料标记，并且在染料和dUTP及UTP之间设计一条特殊的X-linker以确保在酶的作用下更有效的插入DNA或RNA。
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货号 |
产品名称（点击查看详情） |
激发/发射波长(nm) |
规格 |
价格（¥） |
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C401A |
– |
100 µL |
1200 |
|
|
C401B |
– |
1 µmol |
1200 |
|
|
C402A |
– |
100 µL |
1100 |
|
|
C402B |
– |
1 µmol |
1100 |
|
|
C403A |
– |
50 µL |
1800 |
|
|
C403B |
– |
50 nmol |
1800 |
|
|
C404A |
– |
50 µL |
1800 |
|
|
C404B |
– |
50 nmol |
1800 |
|
|
C405A |
495/518 |
25 µL |
1400 |
|
|
C405B |
495/518 |
25 nmol |
1400 |
|
|
C406A |
495/518 |
25 µL |
1800 |
|
|
C406B |
495/518 |
25 nmol |
1800 |
|
|
C407A |
495/518 |
25 µL |
1400 |
|
|
C407B |
495/518 |
25 nmol |
1400 |
|
|
C408A |
495/518 |
25 µL |
1800 |
|
|
C408B |
495/518 |
25 nmol |
1800 |
|
|
C409A |
500/525 |
25 µL |
2800 |
|
|
C409B |
500/525 |
25 nmol |
2800 |
|
|
C410A |
500/525 |
25 µL |
2800 |
|
|
C410B |
500/525 |
25 nmol |
2800 |
|
|
C411A |
553/565 |
25 µL |
2800 |
|
|
C411B |
553/565 |
25 nmol |
2800 |
|
|
C412A |
553/565 |
25 µL |
2800 |
|
|
C412B |
553/565 |
25 nmol |
2800 |
|
|
C413A |
578/602 |
25 µL |
2800 |
|
|
C413B |
578/602 |
25 nmol |
2800 |
|
|
C414A |
578/602 |
25 µL |
2800 |
|
|
C414B |
578/602 |
25 nmol |
2800 |
|
|
C415A |
590/615 |
25 µL |
2800 |
|
|
C415B |
590/615 |
25 nmol |
2800 |
|
|
C416A |
590/615 |
25 µL |
2800 |
|
|
C416B |
590/615 |
25 nmol |
2800 |
|
|
C417A |
650/665 |
25 µL |
2800 |
|
|
C417B |
650/665 |
25 nmol |
2800 |
|
|
C418A |
650/665 |
25 µL |
2800 |
|
|
C418B |
650/665 |
25 nmol |
2800 |
|
|
C419A |
553/565 |
25 µL |
2400 |
|
|
C419B |
553/565 |
25 nmol |
2400 |
|
|
C420A |
553/565 |
25 µL |
2400 |
|
|
C420B |
553/565 |
25 nmol |
2400 |
|
|
C421A |
650/665 |
25 µL |
2400 |
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C421B |
650/665 |
25 nmol |
2400 |
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|
C422A |
650/665 |
25 µL |
2400 |
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C422B |
650/665 |
25 nmol |
2400 |
