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Tonsil Epithelial Cell Medium
(TEpiCM)
Catalog Number: 2561
Product Description
Tonsil Epithelial Cell Medium (TEpiCM) is a complete medium designed for optimal growth of normal human dermal-derived keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of human normal tonsil epithelial cells in vitro.
Components
TEpiCM consists of 500 ml of basal medium, 5 ml of tonsil epithelial cell growth supplement (TEpiCGS, Cat. No. 2572) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503). Note: Due to BPE in the growth supplement, formation of lipoproteins can cause precipitates to be present; the color may vary with different lots.
Product Use
TEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the TEpiCGS and the P/S solution at -20oC. Protect from light.
Shipping
Gel ice.
Prepare for use
Thaw TEpiCGS and P/S solution at 37oC. Gently tilt the TEpiCGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add TEpiCGS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
(TEpiCM)
Catalog Number: 2561
Product Description
Tonsil Epithelial Cell Medium (TEpiCM) is a complete medium designed for optimal growth of normal human dermal-derived keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of human normal tonsil epithelial cells in vitro.
Components
TEpiCM consists of 500 ml of basal medium, 5 ml of tonsil epithelial cell growth supplement (TEpiCGS, Cat. No. 2572) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503). Note: Due to BPE in the growth supplement, formation of lipoproteins can cause precipitates to be present; the color may vary with different lots.
Product Use
TEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the TEpiCGS and the P/S solution at -20oC. Protect from light.
Shipping
Gel ice.
Prepare for use
Thaw TEpiCGS and P/S solution at 37oC. Gently tilt the TEpiCGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add TEpiCGS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.