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Rat Fgb / Fibrinogen beta chain ELISA Kit - Information
The ELISA Genie Rat Fgb / Fibrinogen beta chain ELISA Kit can assay for Rat Fgb / Fibrinogen beta chain in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our Rat Fgb / Fibrinogen beta chain ELISA Kits Work?The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Rat Fgb / Fibrinogen beta chain is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Rat Fgb / Fibrinogen beta chain ELISA Kit - Data
| Description |
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways. |
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| Post-Translational Modification |
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. |
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| Uniprot ID | P14480 |
| Detection method |
Sandwich ELISA Double Antibody |
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| Application |
This immunoassay kit allows for the in vitro quantitative determination of Fgb concentrations in serum plasma and other biological fluids. |
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| Size |
96T |
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| Range |
1.56-100ng/ml |
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| Sensitivity |
< 0.938ng/ml |
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| Storage |
4'C for 6 months |
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| Recovery |
Matrices listed below were spiked with certain level of Fgb and the recovery rates were calculated by comparing the measured value to the expected amount of Fgb in samples.
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| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fgb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) |
Intra-Assay: CV<8% |
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| Note |
For Research Use Only |
