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ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Recommended Procedure For freezedry (lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 12 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Colony and Cell Morphology: After 5 days on YM media colonies butyrous to mycelial and tannishwhite in color. Cells are spheroidal, ellipsoidal to elongate and single, in pairs or small clusters. Pseudohyphae and true hyphae are usually present.
ATCC® Medium 1245: YEPD
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Recommended Procedure For freezedry (lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 12 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Colony and Cell Morphology: After 5 days on YM media colonies butyrous to mycelial and tannishwhite in color. Cells are spheroidal, ellipsoidal to elongate and single, in pairs or small clusters. Pseudohyphae and true hyphae are usually present.