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ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Recommended Procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below 70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally 20°C). Storage of frozen material at this temperature will result in the death of the culture.
1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 23 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Colony and Cell Morphology: On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.510.5 X 36 µm. Pseudohyphae present
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Recommended Procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below 70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally 20°C). Storage of frozen material at this temperature will result in the death of the culture.
1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 23 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Colony and Cell Morphology: On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.510.5 X 36 µm. Pseudohyphae present