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Description
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Strain designation: G00 [ATCC 12996, ATCC 26451, C. Thom 5616.1, CBS 112.46, FGSC A4, Glasgow wild-type, Pontecorvo strain, IMI 38576ii, NRRL 194, SRRC 273, WB 194, Yuill A69, biA-1] Deposited As: Aspergillus nidulans (Eidam) Winter Type strain: No Genotype: argB
Growth Conditions Medium:
ATCC Medium 324: Malt extract agar
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature: 20-25°C
Atmosphere: Aerobic
Handling Procedures For freeze-dry (lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2.From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-5 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Morphology: On PDA medium at 25°C after 6 days, mycelium buff to cinnamon brown becoming army green as conidia develop, velutinous. Reverse tan to reddish brown becoming dark reddish brown. Hyphae hyaline with some stained dark red, guttulate. Conidia globose to subglobose, green, roughened, 4.5-6 X 4.5 µm
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Strain designation: G00 [ATCC 12996, ATCC 26451, C. Thom 5616.1, CBS 112.46, FGSC A4, Glasgow wild-type, Pontecorvo strain, IMI 38576ii, NRRL 194, SRRC 273, WB 194, Yuill A69, biA-1] Deposited As: Aspergillus nidulans (Eidam) Winter Type strain: No Genotype: argB
Growth Conditions Medium:
ATCC Medium 324: Malt extract agar
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature: 20-25°C
Atmosphere: Aerobic
Handling Procedures For freeze-dry (lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2.From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-5 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Morphology: On PDA medium at 25°C after 6 days, mycelium buff to cinnamon brown becoming army green as conidia develop, velutinous. Reverse tan to reddish brown becoming dark reddish brown. Hyphae hyaline with some stained dark red, guttulate. Conidia globose to subglobose, green, roughened, 4.5-6 X 4.5 µm