Use Dispase for gentle dissociation of a wide variety of tissues. Incubation of minced tissue with pre-warmed Dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed Dispase can also be used to harvest cells from tissue culture plastic. This proteolytic dissociation reagent has been optimized for use in the generation of a single-cell suspension from dissociated human and mouse mammary tissue.
This product contains 5 U/mL Dispase II (neutral protease from Bacillus polymyxa) dissolved in Hanks’ Balanced Salt Solution. Unlike trypsin, Dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifugation followed by washing the cells with
buffer or culture medium.