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Description
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C. Recommended Usage For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C. Recommended Usage For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.