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Recombinant Human HMGB1 Protein, CF Summary
Product Specifications
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein
Met1-Glu215
Accession #
N-terminal Sequence
Analysis
Met1 & Gly2
Predicted Molecular Mass
25 kDa
SDS-PAGE
28-34 kDa, reducing conditions
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein
Met1-Glu215
Met1-Glu215
Accession #
N-terminal Sequence
Analysis
Analysis
Met1 & Gly2
Predicted Molecular Mass
25 kDa
SDS-PAGE
28-34 kDa, reducing conditions