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生物试剂
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概述
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
技术规格
| Uses | Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, plasmid construction, and RNA polymerase synthesis of RNA probes and RT-PCR. DNase is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. |
| Stability | When properly stored, this product is stable for 2-3 years. Recommended storage temperature is 2-8°C. |
| Source | Bovine Pancreas |
| Description | Partially purified. A lyophilized powder. |
| Storage | Store at 2-8°C. |
| Activity | ≥2,000 Kunitz units per mg dry weight |
| Unit Definition | 1 Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at our labs. As measured at at our labs, one Kunitz unit digests 1μg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8 in a 50μl reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems. |