Product description:

This reagent detects protein or nucleic acid biological macromolecules by chemiluminescence method. After electrophoresis, the protein or nucleic acid biological macromolecules are transferred to the blotting membrane, and the primary antibody and the horseradish peroxidase (HRP) labeled secondary antibody are used to sequentially bind to the target protein on the membrane; or the target protein labeled with HRP The probe directly or indirectly binds the target nucleic acid on the membrane. After washing the membrane, incubate the membrane with a freshly prepared ECL working solution at room temperature in the dark for 2-3 min. Cover the blot membrane with plastic wrap and fix it on the X-ray film exposure cassette, then transfer it to the dark room to press the X-ray film on The exposure time on the film can be several seconds to several hours. After development and fixation, the protein or nucleic acid bands can be clearly displayed on the X-ray film, or the X-ray film exposure and development step can be omitted, and the blot film can be directly scanned by CCD.