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蛋白名称：Human VEGF-C Protein (His Tag)
蛋白构建：A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his (Cat:10542-H08H) at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc(Cat:10806-H02H), the EC50 of VEGFR3 hFc(Cat:10806-H02H) is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL. 4. Captured VEGFR3/FLT4 Protein, Human (Cat.No.10806-H02H) on anti-Human IgG Fc via CM5 Chip can bind VEGF-C (Cat.No.10542-H08H) with an affinity constant of 31.82 pM as determined in a SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 103
蛋白分子量：The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
蛋白NP号：NP_005420.1
蛋白氨基酸序列：Thr103-Arg227
蛋白标签：C-His
蛋白保存条件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human VEGF-C Protein (His Tag)
蛋白构建：A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his (Cat:10542-H08H) at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc(Cat:10806-H02H), the EC50 of VEGFR3 hFc(Cat:10806-H02H) is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL. 4. Captured VEGFR3/FLT4 Protein, Human (Cat.No.10806-H02H) on anti-Human IgG Fc via CM5 Chip can bind VEGF-C (Cat.No.10542-H08H) with an affinity constant of 31.82 pM as determined in a SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 103
蛋白分子量：The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
蛋白NP号：NP_005420.1
蛋白氨基酸序列：Thr103-Arg227
蛋白标签：C-His
蛋白保存条件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
北京义翘神州科技股份有限公司（简称：义翘神州，品牌：Sino Biological）于2021年8月16日成功登陆深圳证券交易所创业板（股票代码：301047），是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。
义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司，在上海建立分公司，已成为生物试剂行业国内领先的科技公司之一。
凭借多年病毒蛋白研究技术优势，义翘神州仅用53天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究，义翘神州已开发生产近百种新冠病毒相关科研试剂，包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务，可用于筛选出具有中和活性的抗体或血清，支持在细胞水平上的评估。目前，这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。