Directional PCR cloning into the most powerful E. coli expression vectors The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.，官网链接：https://www.sigmaaldrich.cn/product/mm/71071 默克 科研、开发、生产。 作为生命科学行业的全球领先供应商，我们致力于为科研、生物技术开发和生产，以及制药药物疗法开发和生产提供各类解决方案和服务。

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