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Molecular Mass: HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24,8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.
Purity: The purified protein is >95% homogeneous (electrophoresis and mass spectrometry).
Activity: This preparation has low cytokine activity, but is fully active as a DNA binding protein or as an antigen.
Buffer: 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: We advise to keep the product frozen at -20°C.
