Description anti HMGB1 monoclonal antibody The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide P1 (KGKPDAAKKGVVKAEKS). Hybridomas were generated from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1. DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 μg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. This product is intended for research only, and cannot be used on humans.