官网https://www.ptgcn.com/products/TNFAIP3-Antibody-66695-1-Ig.htm
经过测试的应用
Positive WB detected in HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected in rat kidney tissue, human thyroid cancer tissue, mouse kidney tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in HeLa cells
Positive FC (Intra) detected in HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
应用 推荐稀释比
Western Blot (WB) WB : 1:1000-1:6000
Immunohistochemistry (IHC) IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
发表文章中的应用
WB See 2 publications below
IHC See 3 publications below
IF See 2 publications below
产品信息
66695-1-Ig targets TNFAIP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用 WB, IHC, IF
经测试反应性 Human, Mouse, Rat
文献引用反应性 Human, Mouse
免疫原 TNFAIP3 fusion protein Ag18150 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor necrosis factor, alpha-induced protein 3
别名 A20, A20p37, A20p50, EC:2.3.2.-, OTU domain-containing protein 7C
计算分子量 790 aa, 90 kDa
观测分子量 80 kDa
GenBank蛋白编号 BC114480
基因名称 TNFAIP3
Gene ID (NCBI) 7128
RRID AB_2882048
偶联类型 Unconjugated
形式 Liquid
纯化方式 Protein G purification
UNIPROT ID P21580
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件 Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.