官网https://www.ptgcn.com/products/P53-Antibody-60283-2-Ig.htm
经过测试的应用
Positive WB detected in A431 cells, COLO 320 cells, HSC-T6 cells, ROS1728 cells, rat thymus tissue, HaCaT cells, HT-29 cells, HEK-293 cells
Positive IHC detected in human colon cancer tissue, human breast cancer tissue, human gliomas tissue, human lung cancer tissue, human ovary cancer tissue, human ovary tumor tissue, human stomach cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in A431 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.
推荐稀释比
应用 推荐稀释比
Western Blot (WB) WB : 1:5000-1:50000
Immunohistochemistry (IHC) IHC : 1:1600-1:6400
Immunofluorescence (IF)/ICC IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
发表文章中的应用
KD/KO See 5 publications below
WB See 247 publications below
IHC See 31 publications below
IF See 36 publications below
IP See 11 publications below
CoIP See 6 publications below
产品信息
60283-2-Ig targets P53 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用 WB, IHC, IF, IP, CoIP
经测试反应性 Human, Rat
文献引用反应性 Human, Rat, Pig, Rabbit, Canine, Zebrafish
免疫原 P53 fusion protein Ag0698 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor protein p53
别名 TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
计算分子量 44 kDa
观测分子量 53 kDa
GenBank蛋白编号 BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRID AB_2881401
偶联类型 Unconjugated
形式 Liquid
纯化方式 Protein A purification
UNIPROT ID P04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件 Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.