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Fig : Western blot analysis of Met on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10129, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: ACHN cell
Lane 2: PC12 cell
Lane 3: Hela cell
Lane 4: SK-HEP-1 cell
Lane 5: HepA1-6 cell
Lane 6: PANC-1 cell
Lane 7: A431 cell
Predicted molecular weight:156 kDa
Observed molecular weight:~200 kDa
Exposure time：3 min
Positive control:
Lane 1: ACHN cell
Lane 2: PC12 cell
Lane 3: Hela cell
Lane 4: SK-HEP-1 cell
Lane 5: HepA1-6 cell
Lane 6: PANC-1 cell
Lane 7: A431 cell
Predicted molecular weight:156 kDa
Observed molecular weight:~200 kDa
Exposure time：3 min