Catalog No : PMK2116Known As: Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; UrogastronePROPERTIESDescriptionRecombinant Human Epidermal Growth Factor is produced by our E.coliexpression system and the target gene encoding Asn971-Arg1023is expressed.AccessionP01133FormulationLyophilized from a 0.2 μm filtered solution of 20mMTris, 200mMNaCl, pH8.0. Size10µg/50µg/500µg/1mgPurity> 95%Endotoxin< 0.01 EU/µg as determined by LAL test. Predicted Mol Mass6.2 KDaApparent Mol Mass11 KDa, reducing conditionsReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than100μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thawcycles. ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. StorageLyophilized protein should be stored at ≤ -20°C, stable for one year afterreceipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. BackgroundEpidermal growth factor (EGF) is a small 53 amino acid residue longproteinthatcontains three disulfide bridges. It is a small mitogenic proteinthat isthoughttobe involved in mechanisms such as normal cell growth, oncogenesis, andwoundhealing. EGF stimulates the growth of various epidermal and epithelial tissuesinvivo and in vitro and of some fibroblasts in cell culture. This proteinshowsbothstrong sequential and functional homology with human type-alphatransforminggrowth factor (hTGF alpha), which is a competitor for EGF receptor sites. NOTE:The product listed herein is for research use only and is not intended for use in human orclinical diagnosis. Suggested applications of our products are not recommendations to use ourproducts in violation of any patent or as a license. We cannot be responsible for patent infringementsor other violations that may occur with the use of this product.