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生物试剂
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官网https://www.primegene.com.cn/product/16.html
- Synonyms
- Glioma-derived Growth Factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-2, Platelet-derived Growth Factor Beta Polypeptide, Becaplermin
- Accession
- P01127
- GeneID
- 5155
- Source
- Escherichia coli.
- Molecular Weight
- Approximately 24.8 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain).
- AA Sequence
- MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
- Purity
- > 97 % by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Endotoxin
- Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method.
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Stability & Storage
-
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. - Usage
- This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
- SDS-PAGE
-
- Reference
-
1. Hart CE, Bailey M, Curtis DA, et al. 1990. Biochemistry, 29: 166-72.
2. Heidaran MA, Yu JC, Jensen RA, et al. 1992. J Biol Chem, 267: 2884-7.
3. Haber M, Cao Z, Panjwani N, et al. 2003. Vet Ophthalmol, 6: 211-7.
4. Matei D, Kelich S, Cao L, et al. 2007. Cancer Biol Ther, 6: 1951-9.
5. Chung R, Foster BK, Zannettino AC, et al. 2009. Bone, 44: 878-85.
6. Sufen G, Xianghong Y, Yongxia C, et al. 2011. Cell Biol Int, 35: 545-51. - Background
- Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).