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Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.
Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs.
Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs.