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Species | Gene ID | Accession | Source | Length | MW | Tag |
Aequorea victoria | - | AYN77827 | E. coli | 256aa | 28.5kDa | C-PA-Tag |
蛋白信息(About this protein) | |
名称(Name) | Recombinant mEGFP-PA-tag,重组单体增强绿色荧光蛋白融合PA-tag标签 |
别名(Synonyms) | monomeric Enhanced Green Fluorescent Protein with PA-tag; mEGFP-PA-tag; EGFP重组蛋白 |
产品简介(Background) |
Beyotime's Recombinant mEGFP-PA-tag was expressed in E. coli and purified, which contains the full length of mEGFP with PA-tag at the C-terminus. mEGFP is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein (GFP). mEGFP exhibits greater fluorescence stability and brighter than the wild-type GFP. PA-tag is a synthetic peptide consisting of 12 amino acids (GVAMPGAEDDVV). PA-tagged proteins in a dilute sample can be captured by immobilized NZ-1 antibody resin in a near complete fashion and eluted by a solution of free PA14 peptide or 10mM MES pH6.0, 3M MgCl2. The PA tag system proves to outperform many existing affinity tag systems owing to its high affinity, high selectivity, and extended reusability [1]. |
产品用途(Applications) | mEGFP-PA-tag is suitable for testing purifications, detections, and immobilizations with NZ-1 antibody; Protein transfection or microinjection in cells and tissues; Calibration of fluorescent protein detection instrumentation; Western standard. |
外观(Physical appearance) | Liquid |
浓度(Concentration) | 1mg/ml |
纯度(Purity) | >95% by SDS-PAGE |
储存液(Store buffer) | 10mM Tris pH8.0, 150mM NaCl, 1mM EDTA |
本类产品在自然光下呈现绿色(图1左),在紫外光(395-470nm)下呈现绿色荧光(图1右)。

图1.碧云天Recombinant mEGFP类蛋白在自然光与紫外光下效果图。以Recombinant mEGFP-PA-tag (P4405)为例,在自然光下呈现绿色(左),在紫外光(395-470nm)下呈现绿色荧光(右)。实际检测结果可能会因样品和检测条件等的不同而存在差异,图中数据仅供参考。
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。

图2.碧云天Recombinant mEGFP-PA-tag (P4405)的SDS-PAGE电泳检测图。本蛋白(mEGFP-PA)经BeyoGel™ Elite PAGE预制胶(Bis-Tris, 12%, 10孔) (P0849)电泳,Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072),并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际结果会因产品批次、实验条件、检测仪器等的不同而存在差异,图中数据仅供参考。
包装清单:产品编号 | 产品名称 | 包装 |
P4405-50μg | Recombinant mEGFP-PA-tag (1mg/ml) | 50μl |
P4405-200μg | Recombinant mEGFP-PA-tag (1mg/ml) | 200μl |
P4405-1mg | Recombinant mEGFP-PA-tag (1mg/ml) | 1ml |
— | 说明书 | 1份 |
-20ºC保存,一年有效。-80ºC保存,可以保存更长时间。
注意事项:由于蛋白每次冻融均会引起部分失活,所以首次配制成相应浓度储存液后,须分装后-20ºC或更低温度冻存,尽量避免反复冻融。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。