The 17-OH-Progesterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on
the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody (rabbit) directed towards an antigenic site on the
17α-OHP molecule. Endogenous 17α-OHP of a sample competes with a 17α-OHP-horseradish peroxidase
conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase conjugate is inversely proportional to the concentration of 17α-OHP in the
sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the
concentration of 17α-OHP in the sample.