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蛋白名称：Human Glypican 3/GPC3 Protein (Site-Specific AF 647 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human GPC3 (P51654-1) (Met 1-His 559) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主：HEK293 Cells
蛋白纯度：0
蛋白活性：1.Flow cytometric analysis of anti-Glypican 3 CAR expression. 5e5 of anti-Glypican 3 CAR-293 cells were stained with 100 μL of 1 μg/mL of AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated Glypican 3 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 25 & Ser 359
蛋白分子量：The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.
蛋白NP号：P51654-1
蛋白氨基酸序列：Met1-His559
蛋白标签：C-His
蛋白保存条件：Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
蛋白名称：Human Glypican 3/GPC3 Protein (Site-Specific AF 647 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human GPC3 (P51654-1) (Met 1-His 559) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主：HEK293 Cells
蛋白纯度：0
蛋白活性：1.Flow cytometric analysis of anti-Glypican 3 CAR expression. 5e5 of anti-Glypican 3 CAR-293 cells were stained with 100 μL of 1 μg/mL of AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated Glypican 3 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 25 & Ser 359
蛋白分子量：The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.
蛋白NP号：P51654-1
蛋白氨基酸序列：Met1-His559
蛋白标签：C-His
蛋白保存条件：Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
北京义翘神州科技股份有限公司（简称：义翘神州，品牌：Sino Biological）于2021年8月16日成功登陆深圳证券交易所创业板（股票代码：301047），是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。
义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司，在上海建立分公司，已成为生物试剂行业国内领先的科技公司之一。
凭借多年病毒蛋白研究技术优势，义翘神州仅用35天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究，义翘神州已开发生产近百种新冠病毒相关科研试剂，包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务，可用于筛选出具有中和活性的抗体或血清，支持在细胞水平上的评估。目前，这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。