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4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when PNP-alpha-D-Gal is used as substrates.