  High Sensitivity PCR
  High Throughput PCR
  Routine PCR
  AT-rich PCR
  Colony PCR
  Long PCR (up to ~6 kb genomic)

OneTaq^® Quick-Load^® DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep Vent^™

DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure

DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA

Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient

quick-load buffer contains two commonly used tracking dyes for direct loading of PCR products on DNA

gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates

at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli

strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble

material in 30 minutes at 75°C.

5,000 units/ml

1.  Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2.  Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
3.  Powell, L.M. et al. (1987). Cell. 50, 831-840.