The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.
Product Source

An E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.

Reagents Supplied

The following reagents are supplied with this product:

 Applications

• Uracil glycosylase inhibitor (UGI) inhibits uracil-DNA glycosylase (UDG). Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.

Unit Definition

One unit of UGI is defined as the amount of protein required to inhibit one unit of E. coli UDG in 1 hour at 37°C in a total reaction volume of 50 µl. One unit of UDG is the amount of enzyme which will catalyze the release of 60 pmol of uracil per minute from double stranded, uracil-containing DNA.

Reaction Conditions

1X UDG Reaction Buffer
Incubate at 37°C

1X UDG Reaction Buffer:
20 mM Tris-HCl
1 mM DTT
1 mM EDTA
pH 8 @ 25°C

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No 

Materials Sold Separately

UDG Reaction Buffer

Lindahl, T. et al. (1977). J. Biol. Chem.. 252, 3286-3294.

Wang, Z., et al. (1991). Gene. 99, 31-37.

Bennett, S. E. and Mosbaugh, D. W. (1992). J. Biol. Chem.. 267, 22512-22521.