识别位点

概述

TelN Protelomerase, from phage N15, cuts dsDNA at a TelN recognition sequence (56 bp) and leaves

covalently closed ends at the site of cleavage.

An E. coli strain that carries a plasmid with the TelN gene from phage N15.

One unit is defined as the amount of enzyme required to cleave 0.5 μg of pMiniT-TelN BsaI-linearized

control plasmid (313 fmol of TelN recognition sites) in a total reaction volume of 50 μl in 30 minutes at 30°C

in 1XThermoPol Reaction Buffer.

75°C for 5 min