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STEMium™ Human Pluripotent Stem Cell Growth Medium-animal component free
(STEMium-acfTM)
Catalog Number: 5821
Product Description
STEMium-acf™ is a animal component free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. It is made with synthetic and plant-derived components and is free of bovine and other animal-derived components. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. STEMium™ is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes growth of normal human pluripotent stem cells in vitro. This medium should be used in conjunction with Geltrex™ or BD Matrigel™ for feeder-free culture conditions.
Components
STEMium-acf™ consists of 500 ml of STEMium-acf™ basal medium and 10 ml of StemGS-acf® 50X human pluripotent stem cell growth supplement (Cat. No. 5872).
Product Use
STEMium-acf™ is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium and complete STEMium-acf™ at 4°C. Store StemGS-acf® 50X at -20°C. Protect from light.
Shipping
Dry ice.
Prepare for use
Thaw STEMium-acf™ and StemGS-acf® 50X at room temperature. Gently tilt the StemGS-acf® 50X tube several times during thawing to help the contents dissolve. Make sure the contents of the StemGS-acf® 50X are completely dissolved into solution before adding to the STEMium-acf™ basal medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add StemGS-acf® 50X solution into STEMium-acf™ basal medium using sterile techniques, mix well and then the reconstituted medium is ready for use. Since several components of StemGS-acf® 50X are light-labile, it is recommended that the medium not be exposed to light for extended periods of time. If the medium is warmed prior to use, do not exceed 37°C. When stored in the dark at 4°C, complete STEMium-acf™ is stable for two weeks.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
(STEMium-acfTM)
Catalog Number: 5821
Product Description
STEMium-acf™ is a animal component free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. It is made with synthetic and plant-derived components and is free of bovine and other animal-derived components. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. STEMium™ is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes growth of normal human pluripotent stem cells in vitro. This medium should be used in conjunction with Geltrex™ or BD Matrigel™ for feeder-free culture conditions.
Components
STEMium-acf™ consists of 500 ml of STEMium-acf™ basal medium and 10 ml of StemGS-acf® 50X human pluripotent stem cell growth supplement (Cat. No. 5872).
Product Use
STEMium-acf™ is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium and complete STEMium-acf™ at 4°C. Store StemGS-acf® 50X at -20°C. Protect from light.
Shipping
Dry ice.
Prepare for use
Thaw STEMium-acf™ and StemGS-acf® 50X at room temperature. Gently tilt the StemGS-acf® 50X tube several times during thawing to help the contents dissolve. Make sure the contents of the StemGS-acf® 50X are completely dissolved into solution before adding to the STEMium-acf™ basal medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add StemGS-acf® 50X solution into STEMium-acf™ basal medium using sterile techniques, mix well and then the reconstituted medium is ready for use. Since several components of StemGS-acf® 50X are light-labile, it is recommended that the medium not be exposed to light for extended periods of time. If the medium is warmed prior to use, do not exceed 37°C. When stored in the dark at 4°C, complete STEMium-acf™ is stable for two weeks.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.