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Ovarian Epithelial Cell Medium
(OEpiCM)
Catalog Number: 7311
Product Description
Ovarian Epithelial Cell Medium (OEpiCM) is a complete medium designed for optimal growth of normal human ovarian epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human ovarian epithelial cells in vitro.
Components
OEpiCM consists of 500 ml of basal medium, 5 ml of Ovarian Epithelial Cell Growth Supplement (OEpiCGS, Cat. No. 7352) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
OEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the OEpiCGS and the P/S solution at -20oC. Protect from light.
Shipping
Gel ice.
Prepare for use
Thaw OEpiCGS and P/S solution at 37oC. Gently tilt the OEpiCGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add OEpiCGS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
ScienCellResearch LaboratoriesTM
(OEpiCM)
Catalog Number: 7311
Product Description
Ovarian Epithelial Cell Medium (OEpiCM) is a complete medium designed for optimal growth of normal human ovarian epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human ovarian epithelial cells in vitro.
Components
OEpiCM consists of 500 ml of basal medium, 5 ml of Ovarian Epithelial Cell Growth Supplement (OEpiCGS, Cat. No. 7352) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
OEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4oC, the OEpiCGS and the P/S solution at -20oC. Protect from light.
Shipping
Gel ice.
Prepare for use
Thaw OEpiCGS and P/S solution at 37oC. Gently tilt the OEpiCGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add OEpiCGS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
ScienCellResearch LaboratoriesTM