StemDS® Human Embryonic Stem Cell Dissociation Solution
Catalog Number: 5803
Product Description
StemDS® cell dissociation solution is a sterile, phosphate and HEPES-buffered saline solution designed
for the optimal dissociation of adherent human embryonic stem cells. StemDS® solution contains 1 mM
sodium, pyruvate and 10 mM HEPES. This product has a pH of 7.4 at room temperature.
Product Use
StemDS® is used to detach adherent human embryonic stem cells from adherent culture substrates and
is for research use only. It is not approved for human or animal use, or for application in in vitro
diagnostic procedures.
Storage
Store StemDS® at 4°C. Protect from light.
Shipping
Dry ice.
Prepare for use
As with any cell dissociation solution, excessive incubation with StemDS® will damage cell membranes
and will lead to a high level of cell death. The incubation time for cell detachment from the culture
surface is dependent on cell type, population density and substrate; and therefore, should be
determined empirically by individual user. Below is our recommendation for passaging H9 human
embryonic stem cells cultured on BD Matrigel™.
1) Remove medium from culture vessel by aspiration and wash cells twice with Ca+2 and Mg+2 -
free phosphate buffered saline solution to remove cellular debris. Aspirate wash solution.
2) Dispense enough StemDS® into culture vessel to completely cover the cells and place in 37°C
incubator for 3-6 minutes. Colonies should remain adherent after incubation when viewed
under microscope.
3) Aspirate StemDS® and add complete STEMium® growth medium to cells as soon as possible.
4) Cells can be harvested by gently pipetting the cell suspension. Further dilution can be made, if
required, for cell counts and/ or subculturing.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions
when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.