Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Quality Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process Tested and found negative for bacteria, fungi, and mycoplasma Protein concentrations are determined by Lowry method Endotoxin units are measured by Limulus Amoebocyte Lysate assay Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor Source Engelbreth-Holm-Swarm Mouse Tumor Preparation and Storage Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN. Application Cell Growth and Differentiation Metabolism/Toxicology Studies Invasion Assays in vitro and in vivo Angiogenesis Assays in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice Click here for Corning Matrigel Lot Selection and Reservation. For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.