Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Quality Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive PCR testing performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma Protein concentrations determined by Lowry method Endotoxin units measured by Limulus Amoebocyte Lysate assay Gel stability tested for a period of 14 days at 37°C Biological activity determined for each lot using neurite outgrowth assay. Source EHS mouse tumor Application Cell Growth and Differentiation Metabolism/Toxicology Studies Invasion Assays in vitro and in vivo Angiogenesis Assays in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice Preparation and Storage Store at -20°C. Avoid multiple freeze-thaws. Click here for Corning Matrigel Lot Selection and Reservation. For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.