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GeneCopoeia's Lenti-Pac™ HIV Expression Packaging System consists of reagents engineered for efficient, do-it-yourself lentiviral particle prodcution. When combined with GeneCopoeia lentiviral constructs, the results are high viral titers and robust expression levels. The Lenti-Pac™ system includes four products, each available separately.
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- Lenti-Pac™ HIV expression packaging kit. Comes with an optimized lentiviral packaging plasmid mix, eGFP positive control plasmid, EndoFectin™ Lenti, a transfection reagent optimized for virus production, and TiterBoost™ reagent, which increases titers 5-10 fold. This 3rd-generation packaging system ensures high-titer production of particles that are incapable of self-replication.
- Lenti-Pac™ 293Ta packaging cell line. Enables easy and efficient lentiviral plasmid transfection for high-level expression of viral proteins required for packaging.
- Lenti-Pac™ concentration solution. Designed for quick and simple concentration of lentiviral particles. Concentration is achieved by mixing the proprietary reagent with the lentiviral supernatant, followed by a short incubation and centrifugation.
- Lenti-Pac™ titration kit. Designed for quick and simple titration of lentiviral particles. Used for etermining viral copy number and to confirm the success of packaging reactions. Includes reagents fpr RNA extraction and qRT-PCR, as wells as a standard control.