产品描述ABC1 (for ATP-binding cassette transporter 1) is a member of the family of ATP-binding cassette proteins which transport various molecules across biological membranes. ABC1 contains two characteristic ATP-binding domains and 12 transmembrane domains which form a channel-like structure for transport. Mutations in the ABC1 gene are implicated in Tangier disease, characterized by low serum high density lipoprotein. ABC1 is widely expressed in human tissues, with high levels of expression in liver, lung, adrenal glands, placenta and fetal tissue. ABC1 expression is induced during monocyte differentiation and upregulated in the presence of acetylated low-density lipoprotein. ABC1 may have a dual regulatory function in macrophage lipid metabolism and inflammation.
产品名称Anti-ABCA1 Rabbit Polyclonal Antibody
分子量Predicted band size: 254 kDa
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Synthetic peptide within Human ABCA1 aa 21-70 / 2,261.
偶联Non-conjugated
性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Fig1: Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (R1510-42) at 1/500 dilution.
Lane 1: Rat brain tissue lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 254 kDa
Observed band size: 254 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Fig2: Immunocytochemical staining of PC-3M cells using anti-ABCA1 rabbit polyclonal antibody.
Fig3: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-ABCA1 rabbit polyclonal antibody
Fig4: Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-ABCA1 rabbit polyclonal antibody.
Fig5: Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-ABCA1 rabbit polyclonal antibody.
Fig6: Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-ABCA1 rabbit polyclonal antibody.
Fig7: Flow cytometric analysis of Jurkat cells with ABCA1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
背景文献
1. Mostafa, A.M. et al. 2015. Glucagon-like peptide 1 (GLP-1)-based therapy upregulates LXR-ABCA1/ABCG1 cascade in adipocytes. Biochemical and biophysical research communications.
2. Colla o, Rde C. et al. 2012. Protection by Mikania laevigata (guaco) extract against the toxicity of Philodryas olfersii snake venom. Toxicon. 60: 614-622.
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