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The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 μg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 μg of input total RNA in a single 20 μl reaction. Reactions can be scaled up to 100 μl to generate 10 μg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels