产品描述Krüppel-like Factor 2 (KLF2), also known as lung Krüppel-like Factor (LKLF), is a protein that in humans is encoded by the KLF2 gene on chromosome 19. It is a member of the Krüppel-like factor family of zinc finger transcription factors, and it has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. Lung buds removed from KLF2-deficient mouse embryos and cultured from normal tracheobronchial trees. KLF2 is now believed to play an important role in embryonic erythropoiesis, specifically in regulating embryonic and fetal β-like globin gene expression. KLF2 expression is induced by fluid laminar flow shear stress, as is caused by blood flow in normal endothelium. KLF2 has an important function in T-lymphocyte differentiation. T-cells are activated and more prone to apoptosis without KLF2, suggesting that KLF2 regulates T-cell quiescence and survival. KLF2-deficient thymocytes also do not express several receptors required for thymus emigration and differentiation into mature T-cells, such as sphingosine-1 phosphate receptor 1.
产品名称Anti-KLF2 antibody
分子量39 KDa
种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep
验证应用WB,IHC-P,ICC/IF,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human KLF2 251-355/355
偶联Non-conjugated
性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (ER1911-98) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Fig2: Sample:
Lane 1:Brain (Mouse) Lysate at 30 ug
Lane 2:HepG2 Cell Lysate at 30 ug
Primary: Anti-KLF2 (ER1911-98) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :39 kD
Observed band size :39 kD
Fig3: Blank control: A431.
Primary Antibody (green line): Rabbit Anti-KLF2 antibody (ER1911-98)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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