WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Wm: 1:100-1:500. IP: 1:100-1:500. ChIP assay: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
免疫原:
Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human Histone H3. The exact sequence is proprietary.
免疫原种属:
Human
克隆性:
Polyclonal
纯化方式:
Purified by antigen-affinity chromatography.
偶联:
Unconjugated
产品浓度:
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
阳性对照:
4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 (100 ng/ml Nocodazole treatment for 24 hr)
保存温度:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
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