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突变谱均一，可扩增6kb的片段 ；只需改变模板浓度或扩增循环数，即可实现期望的突变频率。突变用酶是Mutazyme I + Taq2000高错误率的DNA聚合酶混合物 GeneMorph II Random Mutagenesis Kit, 30 rxns The GeneMorph II random mutagenesis kit takes error-prone PCR to the next level by combining mutational spectrums of both our Mutazyme DNA polymerase and Taq DNA polymerase. The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries. GeneMorph II kits utilize Mutazyme II DNA polymerase, which is a novel error prone PCR enzyme blend with equivalent mutation rates at A’s and T’s vs. G’s and C’s. Additionally, to address the need for efficient and flexible cloning methods while delivering this less biased mutational spectrum, the GeneMorph II EZClone Domain Mutagenesis Kit offers an easy and fast cloning method to perform targeted random mutagenesis on protein domains and promoter elements and eliminates the need to have specific restriction sites. 特点： Superior yields over a wide target range - 0.1 to 6 kb Easily change mutation frequency by altering concentration of input template Efficient mutagenesis rates of 1 to 16 bases per kb