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millipore，0.5ml，超滤管，分子量：10000
  • 商城价格 登录后可查看价格
  • 货号 UFC5010BK
  • 品牌 Merck millipore/默克密理博 ( 经销商 )
  • CAS号
  • 规格/包装 1个/包
  • 单位 个
  • 储存条件 室温
  • 现货状态 两个工作日
  • 数量

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  • 产品介绍：

  1. 起始容量500ul，最高浓缩至15ul，浓缩倍数可达25倍到30倍

  2. 高达90%的回收率

  3. 10到30分钟完成浓缩过程

    Concentrates 500 µL down to 15 µL

  • Concentration factor 25 x to 30 x

  • High sample recovery (greater than 90% of dilute starting solution)

  • Fast processing time - 10 to 30 minutes

  • Consistent recoveries thanks to the reverse spin capability

  • Reverse spin capability provides consistent recoveries


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Material Supplied

The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – Protein Concentration


Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.

Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.

Typical Concentrate Volume/Concentration Factor vs. Spin Time

Concentrate Volume / Concentration Factor






Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table.

Typical Retention of Protein Markers

Marker/Concentration Molecular Weight Device NMWL % Retention Spin Time (min)
α-Chymotrypsinogen (1 mg/mL) 25,000 3K > 95 30
Cytochrome c (0.25 mg/mL) 12,400 > 95 30
Vitamin B-12 (0.2 mg/mL) 1,350 < 42 30
α-Chymotrypsinogen (1 mg/mL) 25,000 10K > 95 15
Cytochrome c (0.25 mg/mL) 12,400 > 95 15
Vitamin B-12 (0.2 mg/mL) 1,350 < 42 15
BSA (1 mg/mL) 67,000 30K > 95 10
Ovalbumin (1 mg/mL) 45,000 > 95 10
Cytrochorme c (0.25 mg/mL) 12,400 < 35 10
BSA (1 mg/mL) 67,000 50K > 95 10
Ovalbumin (1 mg/mL) 45,000 ~ 40 10
Cytrochorme c (0.25 mg/mL) 12,400 < 20 10
Thyroglobulin (0.5 mg/mL) 677,000 100K > 95 10
IgG (1 mg/mL) 156,000 > 95 10
Ovalbumin (1 mg/mL) 45,000 < 30 10
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12.


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