Cry1 Antibody Rabbit Polyclonal GeneID Antigen Affinity Purified Protein ID Lot No. 1407 NP_004066.1 STORAGE/SHELF LIFE 2 - 8° C / 1 year from date of receipt AMOUNT 100 µl PHYSICAL STATE Liquid PRODUCTION PROCEDURES Antibody was affinity purified using an epitope specific to Cry1 immobilized on solid support. The epitope recognized by A302-614A maps to a region between residue 536 and 586 of human cryptochrome 1 using the numbering given in entry NP_004066.1 (GeneID 1407). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. ORIGIN USA SPECIES REACTIVITY Human Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. APPLICATIONS APPLICATION NOTES Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE (link to IP-western blot protocol in Additional Info section below). Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE. APPLICATIONS WB, IP BUFFER Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide ISOTYPE IgG ADDITIONAL INFO https://www.bethyl.com/product/A302-614A Use the link above to view SDS, a current list of citations, and other product specific information. IP-western blot protocol: https://www.bethyl.com/content/protocol_IP_WB This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc. Eric McIntush, PhD | Chief Scientific Officer Date: June 21, 2019