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经过测试的应用
| Positive WB detected in | U2OS cells, pig brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in |
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) |
WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) |
IHC : 1:250-1:1000 |
| Immunofluorescence (IF) |
IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
