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ChromoTek DYKDDDDK Fab-Trap™ Agarose
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  • 货号 ffa-10
  • 品牌 Proteintech/武汉三鹰 ( 经销商 )
  • CAS号
  • 规格/包装 10rxns
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  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

  • Analysis of unspecific binding of endogenous proteins to the DYKDDDDK Fab-Trap™ in the absence of Flag® fusion protein. Lysates devoid of Flag® fusion protein from HEK cells (HEK293T), Yeast (Saccharomyces cerevisiae), Insect cells (HighFive), Bacteria (Escherichia coli TG1) , and Plant (Arabidopsis thaliana) were subjected to immunoprecipitation with DYKDDDDK Fab-Trap™ and elution with 2x SDS-sample buffer. SDS-PAGE analysis shows elution of Fab-fragment only without contamination of endogenous proteins. L: Prestained protein marker (Proteintech, PL00001), I: Input, B: Bound.

    Analysis of unspecific binding of endogenous proteins to the DYKDDDDK Fab-Trap™ in the absence of Flag® fusion protein. Lysates devoid of Flag® fusion protein from HEK cells (HEK293T), Yeast (Saccharomyces cerevisiae), Insect cells (HighFive), Bacteria (Escherichia coli TG1) , and Plant (Arabidopsis thaliana) were subjected to immunoprecipitation with DYKDDDDK Fab-Trap™ and elution with 2x SDS-sample buffer. SDS-PAGE analysis shows elution of Fab-fragment only without contamination of endogenous proteins. L: Prestained protein marker (Proteintech, PL00001), I: Input, B: Bound.

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