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Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2) and PC-12 (Lane 3). The blot was probed with Anti-LAMP2 Rabbit Polyclonal Antibody (Product # 51-2200, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A ~110 kDa band corresponding to LAMP2 was observed across the cell lines tested.