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Frontiers in immunology 2022 - Cryopreserved NK cells exert antitumor effect in an in vivo PDX mouse model. Ascites were collected from an NSG mouse that had PDX tumor injected intraperitoneally. Spheroids were obtained from the ascites, and 20,000 spheroids were injected in suspension back to the intraperitoneal ( i.p. ) cavity of female NSG mice and allowed to seed for 3 days. Mice were then treated with 2 doses of cryopreserved NK cel