Intended Use
The kit applies for detecting the level of anti-SARS-CoV-2 Omicron (XBB.1.5) Spike RBD antibodies in serum.
Principle of the assay
The principle of the kit is indirect ELISA. SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated HRP-mouse anti-Human IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.
Materials Provided
Components | 1 Kit (96 Tests) |
Microplate: Pre-coated with SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein | 1 Plate |
HRP-mouse anti-Human IgG | 150 μL |
Anti-SARS-CoV-2 Spike Antibody (Control) | 30 mL |
20 × Dilution Buffer | 5 mL |
20 × Wash Buffer | 25 mL |
Color Reagent A | 12.5 mL |
Color Reagent B | 12.5 mL |
Stop Solution | 8 mL |
Storage
Unopened Kit: Store at 2 - 8℃ and the kit is stable for 3 months upon receipt.
Opened/Reconstituted Reagents: Please refer to CoA