c-Fos antibody - 226 308

c-Fos is a member of the transcription factor complex AP1

Guinea pig monoclonal recombinant IgG

Cat. No. 226 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 200 up to 1 : 1000 gallery
Clone	Gp108B5
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity	Reacts with: mouse (P01101), rat (P12841), human (P01100). Other species not tested yet.
Matching control protein/peptide	226-0P
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells. IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.