c-Fos antibody - 226 008
c-Fos is a member of the transcription factor complex AP1
Rabbit monoclonal recombinant IgG
Cat. No. 226 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications

WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 1000 gallery
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery
IHC-P: 1 : 200 up to 1 : 1000 gallery
iDISCO: 1 : 500 gallery
Clarity: 1 : 10000
Clone Rb108B5
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P
Remarks
This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.