搜索

购物车()
首页> 生物试剂


NeuN Rat pAb
  • 商城价格 登录后可查看价格
  • 货号 OB-PRT013-02
  • 品牌 欧赛思生物/Oasis Biofarm ( 经销商 )
  • CAS号
  • 规格/包装 50ul
  • 单位 支
  • 储存条件
  • 现货状态 一周
  • 数量

    - +

    成功收藏产品
  • 立即购买
登录后可见商家信息
热门推荐
商家描述 产品评价(0)
  •  Rat-anti-NeuN/Rbfox3 polyclonal antibody 
    Catalog No: OB-PRT013
    RRID: AB_2934231
    Host Species: Rat polyclonal antibodies
    Important Notice: Antigen retrieval is optional/recommended before immunostaining.
    Gene Synonyms: Fox-3, Hrn, Hrnbp3, Rbfox3, NeuN, Neuna60, RNA binding protein, fox-1 homolog (C. elegans) 3
    Species Reactivity: Mouse, rat, human, dog; Others have not been tested
    Applications: IF/IHC-Fr: 1:500~1000  |  ICC: 1:500  |  WB: 1:2000~5000  |  IHC-P: 1:200~500
    Antigen Retrieval: For an alternative approach, tissue sections could be incubated in 1x Citrate antigen retrieval solution for 30 min at 85~95°C and cool down to room temperature before next procedure.
    Immunogen: Mouse Rbfox3 peptide fused to GST
    Purification: Affinity purification
    General Notes: FOR RESEARCH USE ONLY
    Storage Buffer: pH7.4, PBS, 55mM Tris, 50mM Glycine, ~40% Glycerol, 0.1% potassium sorbate
    Storage: Shipped at 4°C. Store at -20/4°C for months and -80°C for years. Avoid freeze/thaw cycle.
    Background: NeuN protein (the neuronal nuclear protein) is a well-recognized marker of post-mitotic neurons, and is highly conserved during evolution. It has been widely used to assess the differentiation state of neurons during development for more than 20 years [1]. NeuN is also known as RBFOX3, a pre-mRNA alternative splicing regulator [2, 3]. Rbfox3 homozygous mutant mice exhibit reduced brain weight, increased susceptibility to kainic acid-induced seizures, decreased anxiety-related behaviors, and deficits in synaptic transmission and plasticity in the dentate gyrus [4, 5]. In the central nervous system, this antibody can be used as a substitute of the monoclonal NeuN antibody (clone A60) in labeling post-mitotic neurons.
    [1] Mullen RJ, et al. Development. 1992; 116(1):201-11.
    [2] Kim KK, et al. J Biol Chem. 2009; 284(45):31052-61. DIO: 10.1074/jbc.M109.052969
    [3] Dredge BK, et al. PLoS One. 2011; 6(6):e21585. DIO: 10.1371/journal.pone.0021585
    [4] Lin YS, et al. PLoS One. 2016; 11(10):e0164164. DIO: 10.1371/journal.pone.0164164
    [5] Wang H-Y, et al. Sci Rep-Uk. 2015; 5(1):17383. DIO: 10.1038/srep17383
    Key Words: RBFOX3, NeuN, postmitotic neurons, cortex development, neuron differentiation, pan-neural marker
返回顶部